| Site |
Compatible |
|
|
|
| BamHI |
BglII/BclI |
| PvuII |
Any Blunt |
| EcoRI |
MfeI |
| XhoI |
SalI/PspXI |
| AvaIII |
PstI/Sse8387I |
| ClaI |
AsuII/AccI (*)/AclI/AcyI/NarI |
| AgeI |
Cfr9I (SmaI**)/BspMII/BetI/Cfr10I/NgoAIV (NaeI**)/SgrAI |
| NotI |
Bsp120I (ApaI**)/CfrI/XmaIII |
| SacII |
|
| SplI |
Asp718I (KpnI**)/Bsp1407I/TatI |
| StuI |
Any Blunt |
| SphI |
NspI |
| BspMII |
Cfr9I (SmaI**)/AgeI/BetI/Cfr10I/NgoAIV (NaeI**)/SgrAI |
| Sse8387I |
PstI/AvaIII |
| AsuII |
ClaI/AccI (*)/AclI/AcyI/NarI |
| SalI |
XhoI/PspXI |
| AccI |
ClaI/AsuII/AclI/AcyI/NarI |
| HinDII |
Any Blunt |
| BglII |
BamHI/BclI |
| MfeI |
EcoRI |
|
Polylinker structure. Sites present in the polylinker, 5' to 3', are indicated in bold type. Compatible sites are shown on the right. *: only when "MK" from AccI GTMKAC site is CG (K = G or T, M = A or C). **: prototype site with distinct cleavage structure. | |
Martin et al. BMC Biotechnology 2006 6:4 doi:10.1186/1472-6750-6-4 |
|