Table 4 |
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|
Primers and fluorogenic probes for Real-time PCR |
|||||
|
Target/amplicon length |
Specificity/sequence accession number |
Primer and probe names |
Primer/probe sequence |
Lengh (bp) |
Reference |
|
|
|||||
|
Lectin 81 bp |
soybean specific gene (Accession number K00821) |
TM-Lectin-F |
5'-tcc acc ccc atc cac att t-3' |
19 |
29 |
|
TM-Lectin-R |
5'-ggc ata gaa ggt gaa gtt gaa gga-3' |
24 |
|||
|
Lectin-FAM |
5'-FAM-aac cgg tag cgt tgc cag ctt cg-TAMRA-3' |
23 |
|||
|
|
|||||
|
Invertase 79 bp |
maize specific gene (Accession number U16123) |
ivr1-TM1 |
5'-tgg cgg acg acg act tgt-3' |
18 |
30 |
|
ivr1-TM2 |
5'-aaa gtt tgg agg ctg ccg t-3' |
19 |
|||
|
ivr |
5'-VIC-cga gca gac cgc cgt gta ctt cta cc-TAMRA-3' |
26 |
|||
|
|
|||||
|
p35S 82 bp |
p35S promoter (Accession number V00141) |
TM-35S-1 |
5'-gcc tct gcc gac agt ggt-3' |
18 |
29, 31 |
|
TM-35S-2 |
5'-aag acg tgg ttg gaa cgt ctt c-3' |
22 |
|||
|
35S |
5'-FAM-caa aga tgg acc ccc acc cac g-TAMRA-3' |
22 |
|||
|
|
|||||
|
tNOS 151 bp |
tNOS terminator (Accession number V00087) |
tNOSF |
5'-gtc ttg cga tga tta tca tat aat ttc tg-3' |
29 |
32 |
|
tNOSR |
5'-cgc tat att ttg ttt tct atc gcg t-3' |
25 |
|||
|
5'-FAM aga tgg gtt ttt atg att aga gtc ccg caa-TAMRA- |
|||||
|
tNOSpro |
3' |
30 |
|||
|
|
|||||
|
GTS 40-3-2 soybean 83 bp |
construct specific (Accession number AB209952) |
RRS-F |
5'-gcc atg ttg tta att tgt gcc at-3' |
23 |
29 |
|
RRS-R |
5'-gaa gtt cat ttc att tgg aga gga c-3' |
25 |
|||
|
5'-FAM-ctt gaa aga tct gct aga gtc agc ttg tca gcg- |
|||||
|
RRS |
TAMRA-3' |
33 |
|||
|
|
|||||
|
MON810 maize 115 bp |
event specific (Accession number AF434709) |
Mon810F1311 |
5'-cct tca taa cct tcg ccc g-3' |
19 |
33 |
|
Mon810R1311 |
5'-aat aaa gtg aca gat agc tgg gca-3' |
24 |
|||
|
5'-FAM-acg aag gac tct aac gtt taa cat cct ttg cca- |
|||||
|
Mon810pro1311 |
TAMRA-3' |
33 |
|||
|
|
|||||
|
Cankar et al. BMC Biotechnology 2006 6:37 doi:10.1186/1472-6750-6-37 |
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