Differential effect on PCR efficiency of amplicons. The differential influence on PCR reaction for two amplicons is shown for two samples of soybean obtained in routine analyses. Although the same amount of DNA was added to each PCR reaction, inhibition was evident for the lectin amplicon but not for the RRS specific amplicon for a soybean feed sample (A). In the case of soybean flour (B), efficiency of the PCR reaction was lower for the RRS amplicon and differed substantially from that for the lectin amplicon. (k = calculated slope of the standard curve linear regression line).
Cankar et al. BMC Biotechnology 2006 6:37 doi:10.1186/1472-6750-6-37