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Open Access Methodology article

Thin films of Type 1 collagen for cell by cell analysis of morphology and tenascin-C promoter activity

Kurt J Langenbach1, John T Elliott1, Alex Tona2, Dennis McDaniel1 and Anne L Plant1*

Author Affiliations

1 Biotechnology Division/National Institute of Standards and Technology, Gaithersburg, MD 20899, USA

2 Geo-centers, Inc. Newton, MA 02459, USA

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BMC Biotechnology 2006, 6:14  doi:10.1186/1472-6750-6-14

Published: 6 March 2006

Additional files

Additional File 1:

Effect of serum starvation on cell cycle and cell response. A. Flow cytometry of propidium iodide stained cells kept for 16 h in complete medium (left) or in medium with 0.1% serum. The region indicated as 'C' corresponds to G0/G1, 'D' to S and 'E' to G2/M phase of the cell cycle. B. Cell area for cells on thin films of fibrillar collagen (■,□) and thin films prepared from the lower concentration of collagen (●,○). Open circles represent cells that have been serum starved. C. GFP expression on thin films of fibrillar collagen (high) and lower concentration collagen (low). Hatched bars represent cells that have been serum starved.

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Additional File 2:

Live NIH3T3 TN-1/pd2EGFP-1 cells on fibronectin films. Cells were kept at 37°C in an incubator chamber on the microscope stage. Images were taken after cells were fully spread (after at least 2 hours after seeding) at 5 min intervals for a total time of 6 h. Three cells are outlined in several frames in the sequence for ease of viewing.

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