Methodology article

A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production

Lisa D Cabrita, Weiwen Dai and Stephen P Bottomley^*

Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Clayton, 3800, Australia

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BMC Biotechnology 2006, 6:12 doi:10.1186/1472-6750-6-12

Published: 1 March 2006

Additional File 1:

Oligonucleotide sequences used to isolate the genes for GST, MBP and Nus, as well as the annealing and extension times used for amplification.

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