Table 1

STR genotyping performance

gDNA Input (ng)

Completed1

% Completion

No Amplification

GQ<0.252

Concordant

% Concordance

Discordant Genotypes

% Discordance


gDNA

864

100.03

0

13

851

100.04

0

0.0

1

821

95.0

43

70

638

80.1

150

19.0

10

862

99.8

2

9

830

97.1

25

2.9

25

863

99.9

1

22

823

97.7

19

2.3

50

863

99.9

1

15

836

98.6

12

1.4

100

861

99.7

3

11

826

96.8

27

3.2

200

864

100.0

0

0

858

99.3

6

0.7


1N = 27 gDNA or wgaDNA samples were genotyped in duplicate using the AmpFlSTR® Identifiler® assay for N = 864 attempted genotypes/sample. 2A Genotype Quality Score (GQ) of <0.25 indicates a STR genotype with a Genotype Quality Score beloew the calling threshold of GeneMapper v3.0 software. 3gDNA exhibited significantly greater STR genotype completion rate than did 1 ng gDNA input (p < 0.001). 4gDNA exhibited significantly greater STR genotype concordance rates compared to wgaDNA (p < 0.0001, except for 50 ng gDNA input, with p = 0.001, and 200 ng gDNA input, p = 0.03).

Bergen et al. BMC Biotechnology 2005 5:24   doi:10.1186/1472-6750-5-24

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