Figure 2.

Differentiation of PC-12 cells attached to culture dishes coated with PEI. PC-12 cells attached to PEI coated dishes remained firmly anchored to the plate and extended neurites upon treatment with NGF. The pictures show the progress of the differentiation of the treated cells over time: 0 h (panel A), 24 h (panel B), 48 h (panel C) and 96 h (panel D) after the initiation of treatment with 100 ng/ml of NGF. The bar in the photomicrographs corresponds to 25 μm.