GATA transcription factors transactivate Renilla luciferase gene expression in control plasmids. Various Renilla luciferase (Rluc) reporter plasmids were co-transfected into COS-7 cells with expression plasmids encoding the two isoforms of human GATA-6 (hG6-MALT and hG6-MYQ), mouse GATA-6 (mG6), mouse GATA-4 (mG4) or the empty vector pcDNA3. 48 h after transfection, cells were harvested and lysed. Rluc activities were then measured by the Dual Luciferase Reporter assay system and expressed as fold increase compared with control cells transfected with pcDNA3 (mean ± sem of at least 4 experiments). *P < 0.05.
Ho and Strauss BMC Biotechnology 2004 4:10 doi:10.1186/1472-6750-4-10