Table 2 |
|||||
|
Variance component estimates (standard error in parenthesis) for the three DNA quantification methods (four evaluations). |
|||||
|
Variance Component |
N (Replicates) |
OD-U |
OD-D |
QG |
PG |
|
|
|||||
|
Sample |
22 |
0.0064 (0.002) |
0.0067 (0.0021) |
0.0110 (0.0034) |
0.0130 (0.004) |
|
% of Total Variance |
95.7% |
60.0% |
55.0% |
36.0% |
|
|
OD Aliquot |
4 |
0.0000 (0) |
0.0014 (0.0013) |
0.0005 (0.0005) |
0 (0) |
|
% of Total Variance |
0.8% |
12.8% |
2.3% |
0.0% |
|
|
Dilution |
4 |
N/A |
0.0005 (0.0002) |
0.0004 (0.0002) |
0.0011 (0.0004) |
|
% of Total Variance |
Nested w/ OD Aliq. |
4.7% |
1.8% |
3.2% |
|
|
Assay Aliquot |
16 |
N/A |
N/A |
0.0010 (0.0001) |
0.0004 (0.0001) |
|
% of Total Variance |
4.8% |
1.1% |
|||
|
Run |
8 |
0.0000 (0) |
0 (0) |
N/A |
N/A |
|
% of Total Variance |
0.0% |
0.0% |
|||
|
Residual Error |
- |
0.0002 (0) |
0.0025 (0.0001) |
0.0072 (0.0001) |
0.0212 (0.0004) |
|
% of Total Variance |
3.2% |
22.8% |
36.0% |
59.4% |
|
|
Total Variance |
- |
0.0066 |
0.0111 |
0.0201 |
0.0357 |
|
|
|||||
|
Haque et al. BMC Biotechnology 2003 3:20 doi:10.1186/1472-6750-3-20 |
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