Table 2 |
||||||||
|
Summary of transgenic pig analysis. |
||||||||
|
F0 Generation |
F1 Generation |
|||||||
|
|
|
|||||||
|
Animals (F0) |
Strain |
Sex |
Southern* (Tail) |
Southern* (Sperm) |
SEAP Assay** |
Southern (Ratio) |
Positive Pigs |
Littermate |
|
|
||||||||
|
1 |
D |
M |
- |
+ |
||||
|
2 |
D |
M |
- |
- |
||||
|
3 |
D |
M |
- |
+++ |
||||
|
4 |
D |
M |
- |
- |
7/16 |
148–150, 152, 153, 155, 474 |
148–155, 469–476 |
|
|
5 |
D |
M |
+ |
- |
- |
|||
|
6 |
D |
F |
- |
+ |
||||
|
7 |
D |
F |
- |
- |
||||
|
8 |
Y |
M |
- |
- |
- |
1/5 |
187 |
184–188 |
|
9 |
Y |
M |
- |
- |
- |
|||
|
10 |
Y |
M |
- |
- |
+ |
|||
|
11 |
Y |
M |
- |
- |
+ |
0/7 |
138–144 |
|
|
12 |
Y |
M |
- |
- |
- |
|||
|
13 |
Y |
F |
- |
NA |
||||
|
14 |
Y |
F |
- |
+ |
0/12 |
460–468, 477–479 |
||
|
15 |
Y |
F |
- |
+++ |
||||
|
16 |
Y |
M |
- |
+++ |
||||
|
17 |
Y |
M |
+ |
+++ |
||||
|
18 |
Y |
F |
- |
- |
0/10 |
401–410 |
||
|
19 |
Y |
M |
- |
+++ |
||||
|
20 |
Y |
M |
- |
- |
+ |
|||
|
21 |
Y |
M |
- |
- |
+ |
|||
|
22 |
Y |
F |
- |
++ |
0/12 |
215–226 |
||
|
23 |
Y |
F |
- |
+++ |
2/15 |
418, 419 |
411–425 |
|
|
24 |
Y |
M |
- |
++ |
||||
|
25 |
Y |
M |
- |
+ |
+++ |
0/3 |
198–200 |
|
|
26 |
Y |
F |
+ |
++ |
0/10 |
145, 430–438 |
||
|
27 |
Y |
F |
- |
NA |
||||
|
28 |
Y |
F |
- |
NA |
0/8 |
381–388 |
||
|
29 |
Y |
F |
- |
- |
0/2 |
166, 167 |
||
|
30 |
Y |
M |
- |
+ |
- |
|||
|
31 |
Y |
M |
- |
++ |
7/30 |
441, 442, 448, 451, 452, 455, 457 |
439–459, 480–488 |
|
|
32 |
Y |
F |
- |
NA |
||||
|
33 |
Y |
F |
- |
+ |
0/6 |
389–394 |
||
|
34 |
Y |
M |
- |
- |
- |
|||
|
35 |
Y |
M |
- |
- |
+++ |
|||
|
36 |
L |
F |
+ |
NA |
||||
|
37 |
L |
F |
- |
NA |
||||
|
38 |
L |
M |
- |
- |
||||
|
39 |
L |
M |
- |
- |
||||
|
40 |
L |
M |
+ |
NA |
||||
|
41 |
L |
F |
- |
- |
0/9 |
175–182, 197 |
||
|
42 |
L |
F |
+ |
- |
1/5 |
156 |
156–160 |
|
|
43 |
L |
F |
+ |
++ |
1/9 |
206 |
201–209 |
|
|
44 |
D |
M |
+ |
+ |
||||
|
45 |
D |
M |
- |
- |
||||
|
46 |
D |
F |
- |
+ |
||||
|
47 |
D |
F |
- |
NA |
||||
|
48 |
D |
F |
+ |
+ |
||||
|
49 |
D |
F |
- |
+++ |
||||
|
50 |
D |
F |
- |
+++ |
||||
|
51 |
D |
M |
- |
++ |
||||
|
52 |
D |
M |
- |
++ |
||||
|
53 |
D |
M |
- |
+++ |
||||
|
54 |
D |
M |
- |
NA |
||||
|
55 |
D |
F |
- |
+ |
||||
|
56 |
D |
F |
- |
+ |
||||
|
57 |
D |
F |
- |
- |
||||
|
58 |
D |
F |
- |
++ |
||||
|
59 |
D |
M |
- |
+++ |
||||
|
60 |
L |
F |
- |
NA |
||||
|
61 |
L |
M |
- |
NA |
||||
|
62 |
L |
F |
- |
NA |
||||
|
63 |
L |
M |
- |
NA |
||||
|
64 |
L |
M |
+ |
NA |
||||
|
65 |
L |
F |
- |
- |
||||
|
66 |
L |
F |
- |
NA |
||||
|
67 |
L |
F |
- |
NA |
||||
|
68 |
L |
M |
- |
- |
||||
|
69 |
L |
M |
- |
- |
||||
|
70 |
L |
M |
- |
++ |
||||
|
71 |
L |
F |
- |
- |
||||
|
72 |
L |
M |
- |
NA |
||||
|
73 |
L |
F |
- |
- |
||||
|
74 |
L |
M |
- |
- |
||||
|
75 |
L |
M |
- |
+ |
||||
|
|
||||||||
|
D: Duroc; Y: Yorkshire; L: Landrace. *: + or - indicated detectable or undetectable hybridization signal in Southern blot analysis. **: - represents SEAP enzyme activity less than 2 × 104 RLU; + represents SEAP enzyme activity between 2–4 × 104 RLU; ++ represents SEAP enzyme between 4–6 × 104 RLU; +++ represents SEAP activity more than 6 × 104 RLU. |
||||||||
|
Chang et al. BMC Biotechnology 2002 2:5 doi:10.1186/1472-6750-2-5 |
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