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Open Access Highly Accessed Research article

Genetically modified parthenocarpic eggplants: improved fruit productivity under both greenhouse and open field cultivation.

Nazzareno Acciarri1*, Federico Restaino2, Gabriele Vitelli1, Domenico Perrone2, Michela Zottini3, Tiziana Pandolfini3, Angelo Spena3 and Giuseppe Leonardo Rotino4

Author Affiliations

1 Research Institute for Vegetable Crops, Monsampolo del Tronto (AP) Italy

2 Research Institute for Vegetable Crops, Pontecagnano (SA) Italy

3 Dipartimento Scientifico Tecnologico, University of Verona, Verona, Italy

4 Research Institute for Vegetable Crops, Montanaso Lombardo (LO) Italy

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BMC Biotechnology 2002, 2:4  doi:10.1186/1472-6750-2-4

Published: 4 April 2002

Abstract

Background

Parthenocarpy, or fruit development in the absence of fertilization, has been genetically engineered in eggplant and in other horticultural species by using the DefH9-iaaM gene. The iaaM gene codes for tryptophan monoxygenase and confers auxin synthesis, while the DefH9 controlling regions drive expression of the gene specifically in the ovules and placenta. A previous greenhouse trial for winter production of genetically engineered (GM) parthenocarpic eggplants demonstrated a significant increase (an average of 33% increase) in fruit production concomitant with a reduction in cultivation costs.

Results

GM parthenocarpic eggplants have been evaluated in three field trials. Two greenhouse spring trials have shown that these plants outyielded the corresponding untransformed genotypes, while a summer trial has shown that improved fruit productivity in GM eggplants can also be achieved in open field cultivation. Since the fruits were always seedless, the quality of GM eggplant fruits was improved as well. RT-PCR analysis demonstrated that the DefH9-iaaM gene is expressed during late stages of fruit development.

Conclusions

The DefH9-iaaM parthenocarpic gene is a biotechnological tool that enhances the agronomic value of all eggplant genotypes tested. The main advantages of DefH9-iaaM eggplants are: i) improved fruit productivity (at least 30–35%) under both greenhouse and open field cultivation; ii) production of good quality (marketable) fruits during different types of cultivation; iii) seedless fruit with improved quality. Such advantages have been achieved without the use of either male or female sterility genes.