Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies
1 Department of Functional Materials Science, Graduate School of Science and Engineering, Saitama University, 255 Shimo-okubo, Saitama 338-8570, Japan
2 Janusys Corporation, Saitama Industrial Technology Center, 3-12-18 Kamiaoki, Kawaguchi, Saitama 334-0844, Japan
3 Lifetech Co., Ltd., 4074 Miyadera, Iruma City, Saitama 358-0014, Japan
4 Enplas Corporation, 2-30-1 Namiki, Kawaguchi City, Saitama 332-0034, Japan
5 Finetech Corporation, 1-7-1, Asagaya-minami Suginami-ku, Tokyo, Japan
6 Present address: Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet, Bangladesh
7 Present address: Department of Electrical and Electronic Engineering, Tokyo Denki University, 5 Senjyu-Asahi-cho, Adachi-ku, Tokyo 120-8551, Japan
BMC Biotechnology 2014, 14:78 doi:10.1186/1472-6750-14-78Published: 21 August 2014
With the accelerating development of bioscience, the problem of research cost has become important. We previously devised and developed a novel concept microarray with manageable volumes (MMV) using a soft gel. It demonstrated the great potential of the MMV technology with the examples of 1024-parallel-cell culture and PCR experiments. However, its full potential failed to be expressed, owing to the nature of the material used for the MMV chip.
In the present study, by developing plastic-based MMVs and associated technologies, we introduced novel technologies such as C2D2P (in which the cells in each well are converted from DNA to protein in 1024-parallel), NGS-non-dependent microbiome analysis, and other powerful applications.
The reborn MMV-microarray technology has proven to be highly efficient and cost-effective (with approximately 100-fold cost reduction) and enables us to realize hitherto unattainable technologies.