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Open Access Highly Accessed Research article

Positive regulations of adipogenesis by Italian ryegrass [Lolium multiflorum] in 3T3-L1 cells

Soundarrajan Ilavenil1, Mariadhas Valan Arasu15, Jeong-Chae Lee2, Da Hye Kim3, Mayakrishnan Vijayakumar1, Kyung Dong Lee4 and Ki Choon Choi1*

Author Affiliations

1 Grassland and forage division, National Institute of Animal Science, RDA, Seonghwan-Eup, Cheonan-Si, Chungnam 330-801, Korea

2 Research Center of Bioactive Materials, Chonbuk National University, Jeonju 561-756, Republic of Korea

3 The United Graduate School of Agricultural Sciences, Tottori University, Tottori-Shi 680-8553, Japan

4 Department of Oriental Medicine Materials, Dongsin University, Naju 520-714, Korea

5 Department of Botany and microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia

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BMC Biotechnology 2014, 14:54  doi:10.1186/1472-6750-14-54

Published: 11 June 2014

Abstract

Back ground

Intramuscular fat deposition in the meat animal is relatively new strategy for developing the meat quality. Fat deposition is largely depending on the adipocyte proliferation and differentiation. Therefore, we investigated the effect of chloroform extract of L. multiflorum [CELM] on cell proliferation, lipid accumulation and adipocyte differentiation in 3T3-L1 cells and body weight of mouse.

Results

We identified 6,9-Octadecatrienoic acid, Hexadecanoic acid, 2-hydroxypropanoic acid, butane-2,3-diol and hexane-1,2,3,4,5,6-hexaol in CELM. L. multiflorum extract increased the cell viability, lipid accumulation, cell cycle progression and key transcriptional and secretory factors like PPRAγ2, C/CEBP-α, adiponectin, aP2, GLUT-4, FAS and SREBP-1 mRNA expression as compared with control cells. For in-vivo, mice administered with CELM significantly increased body weight throughout the experiment periods. Further, the identified fatty acids like 3, 6, 9-Octadecatrienoic acid and Hexadecanoic acid was docked with target protein [PPRAγ2] using HEX 6.12. The least binding energy considered as high affinity with target protein. The maximum affinity with the target protein was observed in the Hexadecanoic acid followed by 3, 6, 9-Octadecatrienoic acid. The binding efficacy of Hexadecanoic acid and 3, 6, 9-Octadecatrienoic acid to the active site of PPAR-γ2 may be enhanced the adipocyte differentiations.

Conclusion

These findings suggest that CELM stimulates adipogenesis via activating the PPARγ-mediated signaling pathway in adipocyte which could be useful for the development of meat quality in animals.