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Open Access Research article

A novel Streptomyces spp. integration vector derived from the S. venezuelae phage, SV1

Bahgat Fayed1, Ellen Younger2, Gabrielle Taylor1 and Margaret C M Smith1*

Author Affiliations

1 Department of Biology, University of York, York YO10 5DD, UK

2 School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, UK

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BMC Biotechnology 2014, 14:51  doi:10.1186/1472-6750-14-51

Published: 30 May 2014

Abstract

Background

Integrating vectors based on the int/attP loci of temperate phages are convenient and used widely, particularly for cloning genes in Streptomyces spp.

Results

We have constructed and tested a novel integrating vector based on g27, encoding integrase, and attP site from the phage, SV1. This plasmid, pBF3 integrates efficiently in S. coelicolor and S. lividans but surprisingly fails to generate stable integrants in S. venezuelae, the natural host for phage SV1.

Conclusion

pBF3 promises to be a useful addition to the range of integrating vectors currently available for Streptomyces molecular genetics.

Keywords:
Streptomyces; Cloning; Integration vector; Serine integrase; Bacteriophage; SV1