A novel Streptomyces spp. integration vector derived from the S. venezuelae phage, SV1
1 Department of Biology, University of York, York YO10 5DD, UK
2 School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, UK
BMC Biotechnology 2014, 14:51 doi:10.1186/1472-6750-14-51Published: 30 May 2014
Integrating vectors based on the int/attP loci of temperate phages are convenient and used widely, particularly for cloning genes in Streptomyces spp.
We have constructed and tested a novel integrating vector based on g27, encoding integrase, and attP site from the phage, SV1. This plasmid, pBF3 integrates efficiently in S. coelicolor and S. lividans but surprisingly fails to generate stable integrants in S. venezuelae, the natural host for phage SV1.
pBF3 promises to be a useful addition to the range of integrating vectors currently available for Streptomyces molecular genetics.