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Open Access Highly Accessed Open Badges Methodology article

Generation of orientation tools for automated zebrafish screening assays using desktop 3D printing

Jonas N Wittbrodt1, Urban Liebel12 and Jochen Gehrig12*

Author Affiliations

1 Acquifer AG, Karlsruhe, Germany

2 Accelerator Laboratory, Innovation Department, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany

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BMC Biotechnology 2014, 14:36  doi:10.1186/1472-6750-14-36

Published: 1 May 2014

Additional files

Additional file 1:

STL files of orientation tool designs.

Format: ZIP Size: 394KB Download file

Open Data

Additional file 2:

Protocol 1: Step-by-step protocol for generating agarose coated microtiter plates and orientation of embryos using 3D printed orientation tools.

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Additional file 3: Figure S1:

Examples of data from entire 96 well plates containing oriented embryos. Shown are laterally and dorsally oriented embryos from two single 96 well plates. (A-D) lateral orientation and (E-H) dorsal orientation. To illustrate average orientation accuracy within a single plate originating from a routine experiment, embryos were divided in three categories: (A, E) well oriented (lateral: 92.7%, dorsal: 78.1%), (B, F) slightly tilted (lateral: 6.3%; dorsal: 16.7%) and (C, G) failed orientation (lateral: 1.0%; dorsal: 5.2%). Embryos damaged during pipetting or orienting were included in the failed category. Thumbnail images in D and H show overviews of entire 96 well plates.

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Open Data

Additional file 4: Movie S1:

Continuous monitoring of oriented embryos. Shown is a single dorsally oriented embryo from a 96 well plate acquired over a period of 29 hours.

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Open Data

Additional file 5:

OpenSCAD files of orientation tool designs.

Format: ZIP Size: 78KB Download file

Open Data