Generation of orientation tools for automated zebrafish screening assays using desktop 3D printing
1 Acquifer AG, Karlsruhe, Germany
2 Accelerator Laboratory, Innovation Department, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany
BMC Biotechnology 2014, 14:36 doi:10.1186/1472-6750-14-36Published: 1 May 2014
Additional file 1:
STL files of orientation tool designs.
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Additional file 2:
Protocol 1: Step-by-step protocol for generating agarose coated microtiter plates and orientation of embryos using 3D printed orientation tools.
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Additional file 3: Figure S1:
Examples of data from entire 96 well plates containing oriented embryos. Shown are laterally and dorsally oriented embryos from two single 96 well plates. (A-D) lateral orientation and (E-H) dorsal orientation. To illustrate average orientation accuracy within a single plate originating from a routine experiment, embryos were divided in three categories: (A, E) well oriented (lateral: 92.7%, dorsal: 78.1%), (B, F) slightly tilted (lateral: 6.3%; dorsal: 16.7%) and (C, G) failed orientation (lateral: 1.0%; dorsal: 5.2%). Embryos damaged during pipetting or orienting were included in the failed category. Thumbnail images in D and H show overviews of entire 96 well plates.
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Continuous monitoring of oriented embryos. Shown is a single dorsally oriented embryo from a 96 well plate acquired over a period of 29 hours.
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Additional file 5:
OpenSCAD files of orientation tool designs.
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