Table 1

A comparison of the top thirty ranked QTT-A sequences across all amplification experiments
8 bp window sequence Top 30 ranked QTT-A sequences: comparison of rank in other amplification experiments
QTT-A QTT-AR PGT-A PGT-AR QTT-A63 QTT-A65 QTT-A67 QHH-B
GGGGGC GG 1 3 2 3 1 1 1 4180
TTGGGC GG 2 1 3 9 8 5 2 20382
GGGCCG GG 3 12 5 2 5 3 3 606
TGGCCG GG 4 5 4 4 6 6 6 3065
GGTCCG GG 5 6 1 1 3 2 4 1666
GGGTGC GG 6 9 6 13 16 12 9 2377
GTGGGC GG 7 11 10 5 9 7 11 1464
TTGCCG GG 8 8 8 7 22 21 20 13440
GTGCCG GG 9 13 7 8 7 13 17 220
GGGGGC CG 10 19 21 26 2 4 5 3950
TGGTGC GG 11 18 9 14 37 28 19 8530
TTGGGC CG 12 10 19 24 21 27 14 26404
TGCCCG GG 13 14 13 6 13 15 21 3370
GGTCCG GC 14 17 11 12 18 18 23 3802
GGGGGC CA 15 31 24 29 12 14 15 8559
GGGCCG GC 16 32 30 15 10 9 28 2255
TGGCCG GC 17 15 15 16 26 23 22 5312
TTGTGC GG 18 27 23 40 51 69 44 17564
TGTCCG GG 19 21 12 11 32 33 33 9598
TTGGGC CA 20 16 34 35 58 51 47 29598
GGTGGC GG 21 22 16 17 19 22 24 1890
GGGGGC CC 22 36 47 45 15 17 13 10961
TTTGGC GG 23 33 32 22 57 71 39 24345
GGCCCG GG 24 26 14 10 17 16 18 508
TGGGCG GG 25 29 29 23 29 47 40 5616
GGGCGC GG 26 87 33 38 25 34 42 1337
TTGCCG GC 27 23 31 32 46 52 65 18324
TTGGGC GC 28 24 51 57 92 62 60 25137
GGGGGC GC 29 47 58 64 27 24 27 9051
GGGGGC TG 30 35 55 80 4 8 10 8558

The top thirty sequences of the QTT-A touchdown PCR experiments were ranked and compared to the QTT-A repeat experiment (QTT-AR), the PGT-A and PGT-A repeat experiments (PGT-AR), the QTT-A experiments performed at 63°C, 65°C, and 67°C (QTT-A63, QTT-A65, and QTT-A67), and the QHH-B touchdown PCR amplification experiment.

Pan et al.

Pan et al. BMC Biotechnology 2014 14:10   doi:10.1186/1472-6750-14-10

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