Additional file 3: Figure S1.
Design for generating single-primer templates and their amplification. (a) The forward primer consists of a filler sequence, an 8 bp primer testing window and 18 nucleotides specific for a portion of the sense strand of the human IgG C-kappa domain. The reverse primer includes the same filler and 8 bp priming testing site, but includes 18 bp specific for the antisense strand of the kappa domain. After amplification, both the sense and antisense strand contain the same 8 bp sequence for single-primer testing. (b) After the templates are generated, gel purified, and the concentration normalized over all samples, a single primer, which serves as both forward and reverse, consisting of 19 bp of the filler region and 6 bp of the 8 bp priming site, is used in the amplification experiment.
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Pan et al. BMC Biotechnology 2014 14:10 doi:10.1186/1472-6750-14-10