Single primer experiments demonstrating the difference in amplification bias between different polymerase families. For each of the 24 single-primer templates, a subset of sequences for the QHH-B DNA polymerase that have a PPI of the opposite trend with QTT-A DNA polymerase were selected. For agarose gel results, lane 1 shows the QTT-A results while lane 2 shows the QHH-B results, which has PPI values in reverse order. An internal control amplification was also performed on a portion of the sequence consistent with all templates.
Pan et al. BMC Biotechnology 2014 14:10 doi:10.1186/1472-6750-14-10