Table 1

Expression level and regulatory potential of unidirectional and bidirectional vectors
On Off Induction
Construct (rlu/μg) Cells
(x 107) (x103) (x103)
ES.1-Ptet-T6 4.1 ± 0.6 4.1 ± 0.1 9.8 ± 1.5 HeLa-EM2
ES.1-scPtet-T6 1.9 ± 0.2 4.7 ± 0.6 4.0 ± 0.8 HeLa-EM2
MOV-scT6 1.2 ± 0.01 9.2 ± 2.0 1.3 ± 0,3 HeLa

Luciferase activity (rlu/μg protein) was determined after enrichment of transduced Hela-EM2 cells (ES.1 vectors) constitutively providing the M2 transactivator, or Hela cells (MOV-vector) in the presence (“on“) or absence (“off“) of doxycycline (500 ng/ml). Induction was calculated from the activities determined in the on and off state. ± values reflect SEM. For each vector, two populations were established by one round of sorting (pool purity >90%) and were measured twice.

Heinz et al.

Heinz et al. BMC Biotechnology 2013 13:5   doi:10.1186/1472-6750-13-5

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