Table 3

I50 values (in mM) of sodium halides for ChU-B mutant produced in P. pastoris and S. cerevisiae
Inhibitor Substrate pH ChU-B fromP. pastorisI50(mM) ChU-B from S. cerevisiae I50(mM)
NaF ABTS 4.0 0.134 0.109
ABTS 7.4 (blood buffer) 40 42
DMP 5.0 0.174 0.183
NaCl ABTS 4.0 1106 1025
ABTS 7.4 n.m. n.m.
DMP 5.0 931 818

n.m. non-measurable. Inhibition studies were performed at the optimum pH activity value for each substrate tested (4.0 and 5.0 for ABTS and DMP, respectively) and at physiological pH (7.4) with ABTS as reducing substrate.

Mate et al.

Mate et al. BMC Biotechnology 2013 13:38   doi:10.1186/1472-6750-13-38

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