Table 2

Biochemical characteristics of ChU-B mutant produced in P. pastoris and S. cerevisiae
Feature ChU-B fromP. pastoris ChU-B fromS. cerevisiae
Expression levels (mg/L) 43 8
MW1 53,939 53,939
MW2 62,541 60,310
Glycosylation (%) 16 12
N-terminal end ETEAEFSIGP ETEAEFSIGP
Optimum pH (with ABTS) 4 4
Optimum pH (with DMP) 5 5
T50 (°C) 57.9 63.7

1Calculated from the amino acid composition (http://web.expasy.org/compute_pi/ webcite). 2Determined by MALDI-TOF mass spectrometry. The extra N-terminal extension is highlighted in bold.

Mate et al.

Mate et al. BMC Biotechnology 2013 13:38   doi:10.1186/1472-6750-13-38

Open Data