Biochemical characterization of ChU-B mutant. (A) SDS-PAGE: Lane 1, protein standards; lane 2, ChU-B from P. pastoris; lane 3, ChU-B from S. cerevisiae. Samples were resolved on a 12% SDS-polyacrylamide gel and stained with Coomassie Brilliant Blue. (B and C) pH activity profiles. Black circles, ChU-B from P. pastoris; white circles, ChU-B from S. cerevisiae. Activities were measured in 100 mM Britton and Robinson buffer in the pH range from 3.0 to 9.0 with 3 mM DMP (B) or 3 mM ABTS (C) as the reducing substrates. Relative activity (in %) was calculated with respect to the activity at the optimum pH and each point, including the standard deviation, is the average of three independent experiments.
Mate et al. BMC Biotechnology 2013 13:38 doi:10.1186/1472-6750-13-38