Figure 2.

Cloning strategy for the construction of pPICZαChU-B, pGAPZαChU-B, pPICZα*ChU-B and pGAPZα*ChU-B plasmids. The pJRoC30α*ChU-B was used as template to amplify ChU-B with/without the evolved α-factor prepro-leader (α*) from S. cerevisiae. See Methods section for details.

Mate et al. BMC Biotechnology 2013 13:38   doi:10.1186/1472-6750-13-38
Download authors' original image