Mutations in the ChU-B mutant fusion gene. The α-factor pre-leader is depicted in orange, the α-factor pro-leader in blue, and the mature laccase in red. The 15 mutations accumulated in the directed evolution for functional expression in yeast are represented as yellow stars, while the 7 mutations resulted from the evolutionary process for activity in blood are shown as white diamonds. Silent mutations are underlined.
Mate et al. BMC Biotechnology 2013 13:38 doi:10.1186/1472-6750-13-38