Figure 5.

Transcript levels of GFP in leaves of individual transgenic T0 plants with the HPT selectable marker gene controlled by 35S, Nos or tCUP1 promoters. Expression levels are shown on a log scale expressed as 10-ΔCT, where ΔCT is the difference in qRT-PCR threshold cycle number between the respective gene and the reference gene OsActin 1. The results are the mean ± SD of three technical replicates of 20 independent T0 plants randomly selected in each transformation and plotted in order from low to high expression levels.

Zhou et al. BMC Biotechnology 2013 13:29   doi:10.1186/1472-6750-13-29
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