Transcript levels of GFP in leaves of individual transgenic T0 plants with the HPT selectable marker gene controlled by 35S, Nos or tCUP1 promoters. Expression levels are shown on a log scale expressed as 10-ΔCT, where ΔCT is the difference in qRT-PCR threshold cycle number between the respective gene and the reference gene OsActin 1. The results are the mean ± SD of three technical replicates of 20 independent T0 plants randomly selected in each transformation and plotted in order from low to high expression levels.
Zhou et al. BMC Biotechnology 2013 13:29 doi:10.1186/1472-6750-13-29