Sequence of the tCUP1 promoter used in the experiments. The DNA fragment between -394 and +116 of the native tCUP sequence was used as the basal promoter. The transcription start site is shown in bold and numbered with +1.The two upstream ATG codons are indicated in bold with underlining. The translational enhancer element is highlighted by a black box, a Kozak consensus sequence added at the end is shown in bold and italics. This promoter is referred to as tCUP1.
Zhou et al. BMC Biotechnology 2013 13:29 doi:10.1186/1472-6750-13-29