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Open Access Research article

Enhanced transgene expression in rice following selection controlled by weak promoters

Jie Zhou, Yong Yang, Xuming Wang, Feibo Yu, Chulang Yu, Juan Chen, Ye Cheng, Chenqi Yan* and Jianping Chen*

Author Affiliations

State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, MOA Key Laboratory for Plant Protection and Biotechnology, Zhejiang Provincial Key Laboratory of Plant Virology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, P.R.China

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BMC Biotechnology 2013, 13:29  doi:10.1186/1472-6750-13-29

Published: 27 March 2013

Additional files

Additional file 1: Figure S1:

GFP fluorescence in roots of T1 seedlings derived from transformants selected by the HPT gene driven by the 35S (A, B, C, D), Nos (E, F, G, H) and tCUP1 (I, J, K, L) promoters. For 35S, line 17 to 20 (A to D) were selected, for Nos, line 1 (E), 2 (F), 4 (G) and 18 (H) were selected, for tCUP1, line 1 (I), 2 (J), 3 (K) and 20 (L) were selected. Primary roots of 2 T1 seedlings from each of 12 lines (indicated with “*” in Figure 6), were imaged using a fluorescent stereomicroscope under blue light field with the same light intensity and exposure time. Bar= 1 mm.

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Open Data

Additional file 2: Table S1:

Sequences of the primers used in this study.

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Open Data