Enhanced transgene expression in rice following selection controlled by weak promoters
State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, MOA Key Laboratory for Plant Protection and Biotechnology, Zhejiang Provincial Key Laboratory of Plant Virology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, P.R.China
BMC Biotechnology 2013, 13:29 doi:10.1186/1472-6750-13-29Published: 27 March 2013
Additional file 1: Figure S1:
GFP fluorescence in roots of T1 seedlings derived from transformants selected by the HPT gene driven by the 35S (A, B, C, D), Nos (E, F, G, H) and tCUP1 (I, J, K, L) promoters. For 35S, line 17 to 20 (A to D) were selected, for Nos, line 1 (E), 2 (F), 4 (G) and 18 (H) were selected, for tCUP1, line 1 (I), 2 (J), 3 (K) and 20 (L) were selected. Primary roots of 2 T1 seedlings from each of 12 lines (indicated with “*” in Figure 6), were imaged using a fluorescent stereomicroscope under blue light field with the same light intensity and exposure time. Bar= 1 mm.
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