Table 1

Effect of organic solvents on BioHx stability after incubation at 30°C for 1 h
Organic solvents Residual activity (%)
20% 30% 40% 50%
Control 100 ± 0.10 100 ± 0.00 100 ± 0.00 100 ± 0.00
Methanol 94.6 ± 3.2 93.6 ± 4.4 85.5 ± 2.3 57.0 ± 4.0
Ethanol 85.5 ± 0.8 82.2 ± 3.2 77.9 ± 2.1 75.4 ± 4.1
2-Propanol 86.3 ± 3.2 86.3 ± 4.5 76.8 ± 4.4 76.2 ± 3.0
1-Butanol 48.9 ± 3.6 47.9 ± 3.0 30.8 ± 2.7 23.5 ± 2.2
Acetonitrile 74.4 ± 1.2 74.4 ± 3.3 65.0 ± 4.3 44.3 ± 4.5
Acetone 79.6 ± 1.9 78.0 ± 2.7 62.1 ± 3.7 15.9 ± 4.4
DMSO 95.7 ± 2.4 95.3 ± 1.9 54.2 ± 1.0 29.7 ± 3.8
DMF 97.7 ± 0.8 97.3 ± 1.3 72.3 ± 2.1 19.7 ± 1.1

No organic solvent was included in the control samples. The concentrations of organic solvents are indicated (v/v). The enzyme activity was determined under the standard assay condition, and the data is represented as an average of three independent determinations.

Shi et al.

Shi et al. BMC Biotechnology 2013 13:13   doi:10.1186/1472-6750-13-13

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