Effect of metal ions and detergents on BioHx Stability. Aliquots of the enzyme were incubated with each metal ion (10 mM) and detergent (0.5%) at 30°C for 1 h, the residual activity of BioHx was determined using pNP-butyrate as the substrate. All determinants were performed in triplicate.
Shi et al. BMC Biotechnology 2013 13:13 doi:10.1186/1472-6750-13-13