Effect of temperature and pH on BioHx activity and stability. Relative activity of purified BioHx was determined at different temperatures (A) or pH (C) using pNP-butyrate as the substrate at 405 nm. Remaining enzyme activity was measured at 30°C and pH 8.0 after incubating purified BioHx at different temperatures for 30 min (B), or pH at 4°C for 12 h (D). All determinants were performed in triplicate.
Shi et al. BMC Biotechnology 2013 13:13 doi:10.1186/1472-6750-13-13