Open Access Methodology article

A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones

Nisha Hirani1, Marcel Westenberg14, Minaxi S Gami1, Paul Davis2, Ian A Hope3 and Colin T Dolphin1*

Author Affiliations

1 Institute of Pharmaceutical Science, King’s College London, Franklin-Wilkins Building, 150 Stamford Street, London, SE1 9NH, UK

2 EMBL-EBI, Wellcome Trust Genome Campus, Hinxton, Cambridge, CD10 1SD, UK

3 Institute of Integrative and Comparative Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK

4 Present address: Plant Protection Service (NPPO), National Reference Centre, Department of Molecular Biology, P.O. Box 9102, Wageningen, 6700HC, The Netherlands

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BMC Biotechnology 2013, 13:1  doi:10.1186/1472-6750-13-1

Published: 3 January 2013

Additional files

Additional file 1:

Hirani et al Additional methods and data file [[4],[16-20],[43-46]].

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