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Open Access Research article

The Bxb1 recombination system demonstrates heritable transmission of site-specific excision in Arabidopsis

James G Thomson1*, Ronald Chan1, Jamison Smith1, Roger Thilmony1, Yuan-Yeu Yau2, YueJu Wang2 and David W Ow2

Author Affiliations

1 Crop Improvement and Utilization Research Unit, Western Regional Research Center, USDA-ARS, 800 Buchanan Street, Albany, CA 94710, USA

2 Plant Gene Expression Center and UC Berkeley, 800 Buchanan Street, Albany, CA 94710, USA

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BMC Biotechnology 2012, 12:9  doi:10.1186/1472-6750-12-9

Published: 21 March 2012

Additional files

Additional file 1:

Figure S1 Arabidopsis sequences with the highest similarity to Bxb1 attP and attB sites. (a) Alignment of the 48 bp attP site with 18 sequences from the Arabidopsis genome that have 60% or greater overall identity is shown. (b) Alignment of the 42 bp attB site with the nine sequences from the Arabidopsis genome that have 64% or greater identity. (c) Alignment of the 20 bp conserved Bxb1 core att sequence with the five best core matches (2 mismatches to Bxb1 att core) with 50% or greater identity with attP. (d) Alignment of the 20 bp conserved Bxb1 core sequence with the six best core matches (2 mismatches to Bxb1 att core) with 50% or greater identity with attB. Nucleotides identical to the core att site are highlighted in red text, while matches beyond the core are highlighted in blue text, other non-matching sequence is in gray.

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Additional file 2:

Figure 2 Chromosomal alignment of Arabidopsis sequences with the highest similarity to BxbI attP and attB sites. The position and orientation of the 36 att-like sequences are displayed on a diagram of the five Arabidopsis chromosomes. Their orientation is shown with blue (attP) and red (attB) arrowheads.

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Open Data