Additional file 5.
Figure S4. Highly purified full length human recombinant ERCC1 protein used as immunogen. HEK293T cells were transiently transfected by using OriGene ERCC1 TrueORF gold cDNA clone. After transfection, the cells were culture at 37C for another 48 hrs before collection and lysis. The overexpressed recombinant ERCC1 protein was further purified by using anti-DDK affinity column. 0.5ug of purified ERCC1 was loaded for SDS-PAGE analysis.
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Ma et al. BMC Biotechnology 2012 12:88 doi:10.1186/1472-6750-12-88