The production scheme for the protein microarray chip. All the overexpression lysates were produced using OriGene’s TrueORF cDNA clone collection. On a single nitrocellulose slide, over 22,000 protein samples were spotted. These include 10,464 unique gene overexpression lysates printed in duplicate and large selections of positive and negative controls. Since all the expression clones contain a universal Myc and DDK fusion tag, the target gene expression level can be examined by using an Anti-DDK antibody (1:500 dilution of OriGene anti-DDK (TA50011) followed by DyLight 649 conjugated goat anti-mouse IgG secondary antibody for detection.
Ma et al. BMC Biotechnology 2012 12:88 doi:10.1186/1472-6750-12-88