Table 5

Neutralization potential of anti-V3 scFvs against a panel of pseudoviruses and primary isolates
Virus Clade 3E6B 3E7B scFv (HepB) 1418
92RW A 45 >100 >150 >150
SF162 B 115 >100 >150 >150
QZ4589 B 50 >100 >150 >150
AIIMS 261 C 110 45 >150 >150
Du 422 C >150 >100 >150 >150
ZM 53 C >150 >100 >150 >150

The cross neutralizing activities of two anti-V3 scFv clones against a panel of subtype-A, B, C viruses that are listed on the left. The numerical values in the table represent the concentration (μg/ml) of scFvs at which 50% neutralization titers (IC50) was reached. AIIMS 261 is the primary isolate generated in our lab from a HIV-1 clade C infected patient. scFv (HepB) is an scFv against the Hepatitis B surface antigen (negative control) and 1418 is human antibody to parvovirus (negative control). Each experiment was performed at least two independent times.

Kumar et al.

Kumar et al. BMC Biotechnology 2012 12:87   doi:10.1186/1472-6750-12-87

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