Figure 3.

Amplification of VH and VL genes. A. Agarose gel electrophoresis (1%) of PCR amplified products of heavy chain (VH) genes using all combinations of the 24 primers. Lane B, PCR blank (negative control); Lane M, DNA marker (100 bp ladder); Lanes 1–6 is with VH1-6 with reverse primers (R1, R2, R3 and R4). Samples that were loaded in duplicates are indicated as numbers 2, 2 and 3, 3 so on. B. Agarose gel electrophoresis (1%) of PCR amplified products of light chain (VL) genes. Lane B, PCR blank negative control; Lane M, DNA marker (100 bp ladder); Lane 1–6 is with VKL1-6 with reverse primers. C. Pull through PCR for scFv construction, Agarose gel electrophoresis (1%) of PCR amplified products of scFvs constructed by pull through PCR. Lane M, DNA ladder (100 bp); Lanes 1 & 2 are amplified scFv DNA products.

Kumar et al. BMC Biotechnology 2012 12:87   doi:10.1186/1472-6750-12-87
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