Table 2

Lipofection results PKC2109
DNA amount [μg] Plus reagent [μl] Ratio lipid to DNA Quality Cells in suspension Fluor. intensity Transfection efficiency [%]
0.25 0.25 1.0:1 1 0 + 0.6
2.0:1 2 0 + 0.5
2.5:1 2 0 + 3.1
3.0:1 2 0 + 3.8
4.0:1 4 0 ++ 26
0.5 0.5 1.0:1 2 0 + 2.6
2.0:1 4 1 + 26
2.5:1 3 1 ++ 50
3.0:1 5 2 ++ 52
0.5 1.0 2.5:1 4 1 ++ 24
3.0:1 4 2 ++ 45
0.75 0.75 1.0:1 4 0 ++ 28
2.0:1 5 1 ++ 37
2.5:1 5 1 ++ 28
3.0:1 5 1 ++ 21

Scale for the assessment of cell quality: 1- excellent to good; 2- no vacuoles and lipids detectable, cells are bit stretched; 3- partially vacuoles and lipids, stretched cells; 4- many vacuoles and lipids, stressed cells, partially enlarged cells; 5- a lot of large vacuoles and lipids, altered morphology and enlarged cells; Scale for the assessment of cells in suspension: 0- none; 1- low number of cells; 2- a lot of cells; Fluor. = Fluorescence.

Richter et al.

Richter et al. BMC Biotechnology 2012 12:84   doi:10.1186/1472-6750-12-84

Open Data