Table 1

Temperature sensitivity of A/Singapore and B/Vienna viruses in CR.pIX cells
Maximum TCID50 titers [virions/mL]* 33°C 37°C 40°C
Before adaptation to CR.pIX cells
A/Singapore 1.3 × 108 0 -#
B/Vienna 1.8 × 105 0 -
After adaptation to CR.pIX cells
A/Singapore 2.4 × 108 1.8 × 106 0
B/Vienna 3.2 × 106 0 -

*Maximum titers of CR.pIX cells infected in T-flasks at a CCI of 2 × 106 cells/mL and with 1 × 10-6 units trypsin per cell.

#not determined.

Lohr et al.

Lohr et al. BMC Biotechnology 2012 12:79   doi:10.1186/1472-6750-12-79

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