Infection of CR.pIX cells with the LAIV strain A/Singapore at different cell concentrations at time of infection. Viable cell concentrations (A), viabilities (B) and TCID50 titers (C) of bioreactor cultivations are shown. Cells were infected with parameters summarized in Table 2: CCI_4_1 (■, black) and CCI_4_2 (□, black), CCI_6_1 (●, green) and CCI_6_2 (○, green), CCI_8_1 (▼, orange) and CCI_8_2 (∇, orange). After the cell growth phase at 37°C, bioreactor temperature was controlled at 33°C during the infection phase. Error bars for viable cell concentrations and viabilities indicate standard deviations of triplicate measurements.
Lohr et al. BMC Biotechnology 2012 12:79 doi:10.1186/1472-6750-12-79