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Open Access Research article

Live attenuated influenza viruses produced in a suspension process with avian AGE1.CR.pIX cells

Verena Lohr1*, Yvonne Genzel1, Ingo Jordan2, Dietmar Katinger3, Stefan Mahr14, Volker Sandig2 and Udo Reichl15

Author affiliations

1 Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstr. 1, 39106, Magdeburg, Germany

2 ProBioGen AG, Goethestr. 54, 13086, Berlin, Germany

3 Polymun Scientific GmbH, Donaustr. 99, 3400, Klosterneuburg, Austria

4 University for Applied Sciences, Robert-Gerwig-Platz 1, 78120, Furtwangen, Germany

5 Chair of Bioprocess Engineering, Otto-von-Guericke University Magdeburg, Universitätsplatz 2, 39106, Magdeburg, Germany

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Citation and License

BMC Biotechnology 2012, 12:79  doi:10.1186/1472-6750-12-79

Published: 30 October 2012

Abstract

Background

Current influenza vaccines are trivalent or quadrivalent inactivated split or subunit vaccines administered intramuscularly, or live attenuated influenza vaccines (LAIV) adapted to replicate at temperatures below body temperature and administered intranasally. Both vaccines are considered safe and efficient, but due to differences in specific properties may complement each other to ensure reliable vaccine coverage. By now, licensed LAIV are produced in embryonated chicken eggs. In the near future influenza vaccines for human use will also be available from adherent MDCK or Vero cell cultures, but a scalable suspension process may facilitate production and supply with vaccines.

Results

We evaluated the production of cold-adapted human influenza virus strains in the duck suspension cell line AGE1.CR.pIX using a chemically-defined medium. One cold-adapted A (H1N1) and one cold-adapted B virus strain was tested, as well as the reference strain A/PR/8/34 (H1N1). It is shown that a medium exchange is not required for infection and that maximum virus titers are obtained for 1 × 10-6 trypsin units per cell. 1 L bioreactor cultivations showed that 4 × 106 cells/mL can be infected without a cell density effect achieving titers of 1 × 108 virions/mL after 24 h.

Conclusions

Overall, this study demonstrates that AGE1.CR.pIX cells support replication of LAIV strains in a chemically-defined medium using a simple process without medium exchanges. Moreover, the process is fast with peak titers obtained 24 h post infection and easily scalable to industrial volumes as neither microcarriers nor medium replacements are required.

Keywords:
Live attenuated influenza virus; Influenza vaccine production; Suspension cell culture; Cell density effect; AGE1.CR.pIX