Table 5

Cold-induced cell injury to LLC-PK1 cells
LDH release (%)
Solution Without deferoxamine With deferoxamine
L15 45 ± 20 1 ± 0
MEM 35 ± 17 1 ± 1
M199 32 ± 17 1 ± 1
RPMI 40 ± 09 2 ± 0
RPMI + EtOH 38 ± 09
RPMI + tfp + Fructose 09 ± 03
KH 49 ± 13
KH(Ca-,P+) 56 ± 15
KHG 55 ± 13 2 ± 0
KHG(Ca-,P+) 64 ± 14 2 ± 0
KHG(Ca-,P+) + EtOH 63 ± 15
KHG(Ca-,P+) + tfp + Fructose 08 ± 05

LLC-PK1 cells were incubated at 4°C in RPMI 1640, DMEM, L-15, M199, Krebs-Henseleit buffer (KH), KH + glucose (11.1 mM; KHG) and modified KH buffer containing a low Ca2+ concentration and a high inorganic phosphate concentration without (KH(Ca-,P+)) or with glucose (KHG(Ca--,P+)), all with or without the iron chelator deferoxamine (1 mM) for 48 hours. Part of the incubations were performed in the presence of the inhibitors of mitochondrial permeability transition, trifluoperazine (tfp; 20 μM) plus fructose (10 mM). Ethanol was used as solvent control (+ EtOH). Cell injury was assessed by release of lactate dehydrogenase (LDH; n = 4).

Pless-Petig et al.

Pless-Petig et al. BMC Biotechnology 2012 12:73   doi:10.1186/1472-6750-12-73

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