Table 4

Effects of components potentially responsible for the injurious effect of RPMI 1640
LDH release (%)
Without deferoxamine With deferoxamine
KHG 12 ± 08 02 ± 1
KHG + i-inositol 14 ± 09 01 ± 0
KHG + biotin 11 ± 06 02 ± 2
KHG + vitamin B12 12 ± 09 01 ± 1
KHG + p-aminobenzoic acid 13 ± 10 01 ± 0

Components only present in RPMI 1640 or components of RPMI 1640 with concentrations far outside the range of the concentration in other media were added in identical concentration (inositol, 0.2 mM; biotin, 0.8×10-3 mM; vitamin B12, 4×10-6 mM; p-aminobenzoic acid, 7×10-3 mM) to Krebs-Henseleit buffer with 11.1 mM glucose (KHG). Cell injury was assessed by release of lactate dehydrogenase (LDH) at the end of cold incubation (168 h; n = 4).

Pless-Petig et al.

Pless-Petig et al. BMC Biotechnology 2012 12:73   doi:10.1186/1472-6750-12-73

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