Table 3

Composition of different cell culture media and Krebs-Henseleit buffer
RPMI DMEM L-15 M199 KHG
Na+ 139.0 157.0 145.1 144.1 143.6
K+ 5.3 5.8 5.8 5.4 5.9
Mg2+ 0.4 0.8 1.8 0.8 1.2
Ca2+ 0.4 1.8 1.3 1.8 2.5
Cl- 109.5 120.9 146.8 125.8 128.3
SO42- 0.4 0.8 0.8 0.8 1.2
HCO3 - 24.0 44.0 14.3 26.2 25.0
H2PO4-/HPO42- 5.6 0.9 2.0 0.9 1.2
NO3 - 0.9 0.7*10-3 - 4*10-3 -
HEPES - - - - 20.0
Glucose 11.1 25.0 8.3 5.6 11.1
Galactose - - 5.0 - -
Deoxyribose - - - 4*10-3 -
Ribose - - - 3*10-3 -
p-Aminobenzoic Acid 7*10-3 - - 0.4*10-3 -
Biotin 0.8*10-3 - - 4*10-5 -
Choline 20*10-3 29*10-3 10*10-3 4*10-3 -
Folic Acid 2*10-3 9*10-3 2*10-3 2*10-5 -
i-Inositol 0.2 40*10-3 10*10-3 0.3*10-3 -
Niacin - - - 0.2*10-3 -
Niacinamide 8*10-3 33*10-3 10.0*10-3 0.2*10-3 -
Pantothenate 0.5*10-3 8*10-3 4*10-3 2*10-5 -
Pyridoxine 5*10-3 20*10-3 5*10-3 0.1*10 -3 -
Riboflavin 5*10-4 1*10-3 - 3*10-5 -
Thiamine 3*10-3 12*10-3 3*10-3 3*10-5 -
Vitamin B12 4*10-6 - - - -
Glutathione 3*10-3 - - 2*10-3 -
Pyruvate - 1.0 5.0 - -
Phenol red 13*10-3 40*10-3 30*10-3 50*10-3 -
Vitamin A - - - 3*10-5 -
Calciferol - - - 0.3*10-3 -
Menadione - - - 6*10-5 -
α-Tocopherol phosphate - - - 2*10-5 -
Ascorbic acid - - - 0.3*10-3 -
ATP - - - 2*10-3 -
AMP - - - 0.6*10-3 -
Adenine sulfate - - - 30*10-3 -
Guanine - - - 2*10-3 -
Hypoxanthine - - - 2*10-3 -
Thymine - - - 2*10-3 -
Uracil - - - 3*10-3 -
Xanthine - - - 2*10-3 -
Pyridoxal - - - 1*10-4 -
Cholesterol - - - 5*10-4 -
L-Arginine 1.2 0.4 2.5 0.3 -
L-Asparagine 0.4 - 1.9 - -
L-Aspartic acid 0.2 - - 0. 5 -
L-Cysteine - - 1 1*10-3 -
Cystine 0.2 0.2 - 0.1 -
L-Glutamic acid 0.1 - - 0.9 -
L-Glutamine 2.1 4.0 2.1 0.7 -
Glycine 0.1 0.4 2.7 0.7 -
L-Histidine 0.1 0.2 1.6 0.1 -
L-Hydroxyproline 0.2 - - 0.1 -
L-Isoleucine 0.4 0.8 1.0 0.3 -
L-Leucine 0.4 0.8 1.0 0.9 -
L-Lysine 0.3 0.8 0.6 0.4 -
L-Methionine 0.1 0.2 0.5 0.2 -
L-Phenylalanine 0.1 0.4 0.8 0.3 -
L-Proline 0.2 - - 0.4 -
L-Serine 0.3 0.4 1.9 0.5 -
L-Threonine 0.2 0.8 2.5 0.5 -
L-Tryptophan 25*10-3 0.1 0.1 0.1 -
L-Tyrosine 0.1 0.4 1.7 0.2 -
L-Valine 0.2 0.8 0.9 0.4 -

All concentrations are given in mM. Concentrations of RPMI components printed in italics are outside the range of concentrations in the other media (cell culture media, Krebs-Henseleit buffer with 11.1 mM glucose (KHG)), bold printing highlighting the most likely culprits for the enhanced cold-induced cell injury in RPMI.

Pless-Petig et al.

Pless-Petig et al. BMC Biotechnology 2012 12:73   doi:10.1186/1472-6750-12-73

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