Table 1

Comparison of RPMI 1640 from two different companies
LDH release (%)
Conditions of cold incubation RPMI (Gibco) RPMI (Sigma)
No Inhibitor 92 ± 6 89 ± 4
+ Deferoxamine 01 ± 1 02 ± 0
+ Trifluoperazine + fructose 01 ± 1 01 ± 1
+ Ethanol (solvent control) 90 ± 1 88 ± 4

Vero-B4 cells were incubated in RPMI 1640 purchased from Gibco or Sigma at 4°C for 168 h. The iron chelator deferoxamine (1 mM) or trifluoperazine (20 μM) plus fructose (10 mM), the latter as inhibitor combination of mitochondrial permeability transition [30], were added to part of the incubations during cold storage. Ethanol was used as solvent control for trifluoperazine. Cell injury was assessed by release of lactate dehydrogenase (LDH) after cold incubation (n = 4).

Pless-Petig et al.

Pless-Petig et al. BMC Biotechnology 2012 12:73   doi:10.1186/1472-6750-12-73

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