Table 1

Yields (in p.f.u./mL) from cells transfected with partial complements of the eight PR8 rescue plasmids
"4+4"1 "7+1"1
with B12 with Env2 with B12 with Env2
Repeat 1 0 5000 0 1400
Repeat 2 0 390 10 50
Repeat 3 0 110 0 150

This table shows the viral yields (p.f.u./mL) from cells transfected with the eight PR8 rescue plasmids split into separate batches at the time of transfection and brought together on the next day.

1 In the "4 + 4" experiment, one batch of cells was transfected with plasmids encoding segments 1, 2, 3, 5, while a complementary batch of cells was transfected with plasmids encoding segments 4, 6, 7, 8. In the "7 + 1" experiment, one batch was transfected with plasmids encoding segments 1, 3, 4, 5, 6, 7, 8 and the complementary batch was transfected with segment 2. The cells from complementary batches were dislodged and brought together at day 1 post transfection. These experiments consisted of triplicate transfections for each condition (Repeats 1 to 3).

2 In addition to the transfection with influenza plasmids, cells were transfected with either the Env-expressing or the empty B1 plasmid.

Bourret et al.

Bourret et al. BMC Biotechnology 2012 12:69   doi:10.1186/1472-6750-12-69

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