Figure 4.

Effect of adding Env-expressing or inert plasmid beyond 0.8 μg total DNA on primary rescue yields. This figure shows the effect of adding increasing amounts of Env-expressing or inert B1 plasmid to a fixed amount of 0.6 μg of PR8 rescue plasmids on primary rescue efficiency in a 24-well plate format. Each point shows the average yield from three replicate transfections and error bars indicate standard deviation.