Principle of the fusion approach applied to the influenza A 8-plasmid rescue system. The eight virus rescue plasmids are transfected in 293 T cells together with the Env expressing plamsid. Our hypothesis is that individual 293 T cells tend to be transfected with partial complements of the viral rescue plasmids. Upon fusion, all eight necessary viral rescue plasmids can be expressed in one syncytium, allowing rescue of complete virions.
Bourret et al. BMC Biotechnology 2012 12:69 doi:10.1186/1472-6750-12-69