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Open Access Open Badges Methodology article

In-house preparation of hydrogels for batch affinity purification of glutathione S-transferase tagged recombinant proteins

Jason S Buhrman1, Jamie E Rayahin1, Melanie Köllmer1 and Richard A Gemeinhart123*

  • * Corresponding author: Richard A Gemeinhart

Author affiliations

1 Department of Biopharmaceutical Sciences, University of Illinois, Chicago, IL, 60612-7231, USA

2 Department of Bioengineering, University of Illinois, Chicago, IL, 60607-7052, USA

3 Department of Ophthalmology and Visual Science, University of Illinois, Chicago, IL, 60612-4319, USA

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Citation and License

BMC Biotechnology 2012, 12:63  doi:10.1186/1472-6750-12-63

Published: 18 September 2012



Many branches of biomedical research find use for pure recombinant proteins for direct application or to study other molecules and pathways. Glutathione affinity purification is commonly used to isolate and purify glutathione S-transferase (GST)-tagged fusion proteins from total cellular proteins in lysates. Although GST affinity materials are commercially available as glutathione immobilized on beaded agarose resins, few simple options for in-house production of those systems exist. Herein, we describe a novel method for the purification of GST-tagged recombinant proteins.


Glutathione was conjugated to low molecular weight poly(ethylene glycol) diacrylate (PEGDA) via thiol-ene “click” chemistry. With our in-house prepared PEGDA:glutathione (PEGDA:GSH) homogenates, we were able to purify a glutathione S-transferase (GST) green fluorescent protein (GFP) fusion protein (GST-GFP) from the soluble fraction of E. coli lysate. Further, microspheres were formed from the PEGDA:GSH hydrogels and improved protein binding to a level comparable to purchased GSH-agarose beads.


GSH containing polymers might find use as in-house methods of protein purification. They exhibited similar ability to purify GST tagged proteins as purchased GSH agarose beads.

Glutathione; PEGDA; Glutathione S-transferase; Batch purification; Recombinant protein