Flow cytometric analysis of competitive binding of the scFv-Fcs with anti-CD9 mAb, MM2/57. MM2/57 antibody was incubated with MKN-28 cells in the presence (closed histogram) and absence (open histogram) of the indicated scFv-Fcs. Bound MM2/57 was detected by FITC-conjugated goat anti-mouse IgG antibody. Histograms drawn in dotted line in the far left represent the data acquired by the FITC-conjugated secondary antibody only.
Yoon et al. BMC Biotechnology 2012 12:62 doi:10.1186/1472-6750-12-62