Immunofluorescence analyses of the effect of [V7]20-, [RGD-V6]20-, and fibronectin-coated surfaces on the N2a differentiation into TuJ1-positive neurons. N2a cells were cultured in the presence of 20 μM RA treatment (a-c) or absence of RA (d-h). Protein coating concentration was 0.1 μM (b, c,e and f) or 5 μM (g and h). Green and red colors originate from the Alexa Fluor 488-labeled and Alexa Fluor 568-conjugated anti-TuJ1 antibody, respectively. Blue color corresponds to DAPI. Scale bar is 50 μm. Graph (i) compares the percentages of neuritogenesis on TCPS (1), 0.1 μM [V7]20 (2), 0.1 μM [RGD-V6]20 (3), 5 μM [RGD-V6]20 (4) and 5 μM fibronectin (5). * and # represent P < 0.05 and P < 0.01 from TCPS and [V7]20, respectively.
Jeon et al. BMC Biotechnology 2012 12:61 doi:10.1186/1472-6750-12-61